عنوان فارسی مقاله: ارزیابی میزان سمی بودن پلی پلکس chitosan-pDNA در شرایط آزمایشگاهی و طبیعی
عنوان انگلیسی مقاله:
فهرست مطالب
An evaluation of in vitro and in vivo toxicity of chitosan-pDNA polyplexes
Gene delivery to lung
Cationic polymers:DNA complex
Bacterial plasmids
Objectives
Preparation of the chitosan/pDNA polyplex
Characterization
Gel Retardation Assay
Objectives
Cell lines
Cationic polymers:DNA complex
Transfection efficiency
Transfection experiment
Luciferase expression
MTS assay
Cytotoxicity assay: MTS
MTS assay (vary conc)
MTS assay
In vivo study
Luciferase expression in mice lungs
Number of cells in BAL fluids
Total protein and LDH activity
Cytokines
Conclusion
Future work
بخشی از مقاله
Gene delivery to lung
The transmission of DNA encoding for therapeutic gene or protein into the target cells for prevention or treatment of disease
Potential treatment for
Cystic fibrosis, Asthma
Advantages
Easily accessible via airways
Large surface area for transfection
Reduces the risk of systemic side effects
Disadvantages
Repulsion between DNA & cell membrane due to same charge
Enzymatic degradation
کلمات کلیدی:
Gene delivery by lipoplexes and polyplexes. - NCBIhttps://www.ncbi.nlm.nih.gov/pubmed/20359532by IC Tros - 2010 - Cited by 375 - Related articlesEur J Pharm Sci. 2010 Jun 14;40(3):159-70. doi: 10.1016/j.ejps.2010.03.019. Epub 2010 Mar 30. Gene delivery by lipoplexes and polyplexes. Tros de Ilarduya ...Polyplex - Wikipediahttps://en.wikipedia.org/wiki/PolyplexPolyplex are manufacturers of Biaxially Oriented Polyester (BOPET) Film for packaging, electrical and other industrial applications. It has manufacturing facilities ...Images for polyplexesImage result for polyplexesImage result for polyplexesImage result for polyplexesImage result for polyplexesImage result for polyplexesMore images for polyplexesReport imagesLipoplexes and polyplexes - The vehicles of Gene Delivery - SlideSharehttps://www.slideshare.net/salehsarwar1/lipoplexes-and-polyplexes-35211860May 28, 2014 - Lipoplexes and polyplexes - The vehicles of Gene Delivery. 1. Gene Therapy: Treatment which cure disease By introducing DNA into cells ...Nucleic Acid Therapeutics Using Polyplexes: A Journey of 50 Years ...pubs.acs.org/doi/abs/10.1021/cr5006793by U Lächelt - 2015 - Cited by 100 - Related articlesApr 15, 2015 - Poly(ethylene glycol) Crowding as Critical Factor To Determine pDNA Packaging Scheme into Polyplex Micelles for Enhanced Gene ...DNA Polyplexes as Combinatory Drug Carriers of Doxorubicin and ...pubs.acs.org/doi/abs/10.1021/mp500873kby HC Kang - 2015 - Cited by 10 - Related articlesJul 1, 2015 - Compared with the free drug, the polyplexes (100–170 nm in size) delivered more drug into the cytosol and the nucleus and demonstrated ...PEG stabilized DNA – poly(ferrocenylsilane) polyplexes for gene ...pubs.rsc.org › Journals › Chemical Communicationsby B Zoetebier - 2016 - Cited by 1 - Related articlesPolycationic poly(ferrocenylsilane)s (PFS) with tunable amounts of PEG side chains were used for the condensation of DNA into polyplexes of 110 nm in 5.0 mM ...Searches related to polyplexespolyplexes definitionlipoplexes definitiondifference between lipoplex and polyplexlipoplex wikipedialipoplexes gene therapylipoplex gene deliverypolyplex gene deliverylipoplex structure
