عنوان مقاله
افت مخصوص هتروزایگوسیتی (ناخالصی) سرطان خون MHC در بیمار CLL: زمان بندی اثرات وضعیت بیماری از تیپ بندی تاییدی
فهرست مطالب
چکیده
مقدمه
مطالعه موردی
روش ها
نتایج
بحث
بخشی از مقاله
تجزیه تحلیل و آرایه VNTR و aCGH-SNP
محصولات PCR نشان گذاری شده فلورسانت در هشت مکان ژنی (D11S554, D12S391, D16S539, FGA, Penta E, SE33, THO, VWF-B) با استفاده از الکتروفورز با مقدار نسبی هر فرآورده تحت فلورسانس مورد اندازه گیری قرار گرفتند. آزمایش میکروآرایه CGH روی پلاتفرم هدف یابی سرطانG3 CGH-SNP 4×180 K (فناوری های Agilent، سانتا کارلا، CA، USA) حاوی تقریبا 120000 پروب CGH انجام شد که بیش از 500 ژن سرطان و بیش از 130 منطقه ژنومی مربوط به سرطان و 60000 پروب SNP را پوشش می دادند.
کلمات کلیدی:
Leukemia specific loss of heterozygosity of MHC in a CLL patient: Disease state impacts timing of confirmatory typing Myra Coppage a,⇑ , Anwar Iqbal a , Ausaf Ahmad a , Michael W. Becker b aDepartment of Pathology and Laboratory Medicine, University of Rochester Medical Center and the Wilmot Cancer Center, Rochester, NY, United States bDepartment of Medicine Hematology/Oncology, University of Rochester Medical Center and the Wilmot Cancer Center, Rochester, NY, United States article info Article history: Received 22 March 2012 Accepted 3 October 2012 Available online 17 October 2012 abstract A 63 year old white male with refractory B-CLL presented for allogeneic HSCT evaluation; HLA typing was performed on PBL at time of WBC = 53 K, ALC = 47 K and revealed homozygosity at Class I locus and heterozygosity at Class II locus. Two siblings were full mismatches with the recipient and an unrelated search initiated. The patient was treated with Fludaribine and Rituxan complicated by aplastic anemia and bacteremia. Prior to transplant, confirmatory typing performed on PB revealed two full haplotypes at Class I and II. Sample identification error and the presence of third party lymphocyte engraftment as a result of prior red cell or granulocyte transfusion(s) were ruled out by STR analysis of 8 loci of all samples, T and B cells from cryopreserved PB at blast crisis were HLA typed independently. T cell typing yielded both complete haplotypes (genotype verified by offspring HLA typing); B cells typed for homozygous haplotype indicating loss of heterozygosity of MHC locus. Microarray based comparative genomic hybridization of tumor cells confirmed LOH at 6p.
